一种合成的纳米粒高分子材料的细胞毒性研究

Investigation on the Cytotoxicity of Synthetic D,L-lactic and Glycolic Acid/poly (Ethylene Glycol) (PELGE) Nanoparticles.

目的检测本实验室新合成的准备应用于静脉注射的系列高分子材料poly(ethylene glycol)-D,L-lactic and glycolic acid-poly(ethylene glycol)(mPEG-PLGA-mPEG,PELGE)以及由其制成的纳米粒对多种细胞的细胞毒性。方法利用快速评定细胞增殖率和细胞毒性的噻唑蓝比色法(MTT),不仅参照美国药典用小鼠成纤维细胞L929细胞株进行评价,还根据该材料将来可能的应用领域,用Chang氏正常肝细胞株,原代人肾血管内皮细胞,原代人胚成骨细胞,原代人胚成肌细胞评价其细胞毒性。结果结果表明该系列聚合物对L929细胞株24 h内和内皮细胞48 h内的毒性为0或Ⅰ级,即无细胞毒性,而对其它不同种细胞具有不同程度的细胞毒性。结论该材料具有应用于静脉注射的可能性。该实验为进一步的动物试验提供依据,同时为该新型材料应用于实际提供了前提检测方法。

Objective To test the cytotoxicity of synthetic poly （ethylene glycol）-D, L-lactic and glycolic acidpoly （ethylene glycol） （mPEG-PLGA-mPEG, PELGE） and the nanoparticles made of it in our lab for intravenous injection. Methods MTr-assay was used to evaluate cell relative proliferation rate （RGR） and cytoxicity of PELGE eluent. Not only US pharmacopoeia （USP） was consulted with L-929 mouse fibroblasts, but also according to the fields of these materials could be applied other kinds of cell lines or primaly ceils, such as Changg normal hepatic cell lines, human primary renal vascular endothelial cells, human embryonic primary osteoblast and primary human embryonic primaiy myoblast were investigated. Results It was indicated that some RGR induced by PELGE was high, and the cytotoxicity was 0 or I grade according to the USP for L929 during 24 h and vascular endothelial cells during 48 h, meaning that the nanoparticles had no cytotoxieity. However, these kinds of nanoparticles had different effect on the proliferation of the other cells. Conclusion The nanoparticles have potential to be used in vein. And this research is of great help for the further animal experiment and provides an effective testing means for the practice use of PELGE.