二苯甲酰甲烷对对乙酰氨基酚所致MT(-/-)小鼠急性肝损伤的保护作用

Hepatoprotective effect of dibenzoyl methane against paracetamol-induced acute liver damage in MT(-/-) mice

目的探讨二苯甲酰甲烷(DBM)对对乙酰氨基酚所致MT(-/-)小鼠急性肝损伤的保护作用。方法采用对乙酰氨基酚(450mg·kg-1,sc)所致小鼠急性肝损伤模型。雌性MT(-/-)小鼠随机分为5组,对照组、模型组、DBM50,100和200mg·kg-1组。DBM组分别igDBM50,100和200mg·kg-1·d-1,共4d。第4天给药后30min,模型组和DBM组小鼠sc对乙酰氨基酚造模。24h后,测定血清中谷丙转氨酶(GPT)、谷草转氨酶(GOT)和乳酸脱氢酶(LDH)的活性。制备肝匀浆,测定肝中还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)和丙二醛(MDA)的含量;留取肝脏组织,常规石蜡包埋切片,HE染色,光镜观察肝脏组织病理变化。结果与模型组相比,各DBM组小鼠血清中GPT,GOT和LDH活性显著降低;GSH/GSSG比值升高,MDA含量下降;肝脏组织病理损伤明显减轻。结论DBM对对乙酰氨基酚引起的MT(-/-)小鼠急性肝损伤具有明显的保护作用。

AIM To evaluate the hepatoprotective effect of dibenzoylmethane （ DBM ） on paracetamol-induced liver damage in MT （-/-） mice. METHODS Fifty MT（-/-） mice were divided into 5 groups： the normal control group, paracetamol model group, and DBM 50,100 and 200 mg.kg-t groups. The mice in DBM groups were administered ig DBM 50, 100 and 200 mg·kg^-1·d - 1, respectively, for 4 d. The control group and model group were given normal saline. On the 4th day, paracetamol 450 mg·kg^-1 was given sc to all groups except the control group 30 min after DBM administration. The mice serum was prepared and the mice were then euthanized 24 h after paracetamol administration. The serum activities of GPT, GOT and lactic dehydrogenase （LDH） were determined and the liver tissues were col- lected for histopathological assessment under light microscope. The levels of glutathione （ GSH ）, glutathione disulfide （ GSSG ） and malondialdehyde （MDA） in liver homogenate were also quantified. RESULTS Compared with model group, the serum GPT, GOT and LDH activities in DBM groups were significantly reduced. HE staining showed that liver inju- ry in DBM groups was improved and the ratio of GSH/GSSG in liver homogenate was increased, and the content of MDA was markedly reduced too. CONCLUSION DBM has an obvious protective effect against paracetamol-induced acute heDatotoxicitv in MT（-/-） mice.