摘要
目的建立犬STR复合扩增体系，为犬个体识别和亲权鉴定提供一种检测方法。方法用自行建立的11个犬STR基因座复合扩增体系，使用AB13130XL遗传分析仪，对107份藏獒唾液DNA样本的扩增产物进行检测及统计学分析。结果除1个基因座杂合度低于60％，其他10个基因座杂合度均高于70％；11个基因座PIC值均在0．6以上；11个基因座中有8个基因座的个体识别率在0．938以上，其余3个基因座均在0．826以上；父权排除率除TETRA为0．267，其余在0．401—0．749之间，偶合机率1．08×10^-18。结论此11个犬STR基因座在藏獒犬中具有较高的个体识别能力，可用于犬类个体识别和亲权鉴定。
Objective To develop a multiplex PCR system of canine STR for individual identification and paterntity testing of dogs. Methods 107 tibetan mastiffs were genotyped by using the self-constructed multiplex PCR amplification system containing 11 canine STR loci with ABI 3130 genetic analyzer. Results In addition to one locus less than 60%, the heterozygosity of other 10 loci was higher than 70%, and the polymorphism information content （PIC） of all of 11 loci was more than 0.6. The discriminating power（DP） of these 11 loci was more than 0.826, in which nine loci surpassed 0.938. The probability of exclusion（EP） for each locus ranged from 0. 401 to 0. 749 except TETRA locus which the EP was 0.267. The match probability for these 11 loci was estimated to be 1.08 × 10^-13. Conclusion These 11 dog-STR loci are highly polymorphic and suitable for individual identification and paternity testing.
出处
《中国法医学杂志》
CSCD
北大核心
2009年第1期39-42,共4页
Chinese Journal of Forensic Medicine
关键词
法医物证学
藏獒犬
个体识别
亲权鉴定
forensic biological evidence
tibetan mastiff
individual discrimination
paternity identification
