摘要
Objective： To screen and identify key genes differentially displayed in mouse fore stomach carcinoma, in order to elucidate the molecular mechanism underlying carcinogenesis. Methods： The animal models complied with each period of NIH mouse fore stomach carcinoma induced by N-Nitrososarcosineethylester （NSEE） were used in this study. The mice were euthanized on days 14, 28, 56, 77 and 84, respectively, after NSEE-piped treatment, and classified according to their pathologies. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential display technique and screened by using Reverse Northern blot. Bioinformatics were employed to analyze the results observed. After identification, ten fragments were cloned and matched with GENEBANK database through homologous analysis. Results： One gene was found identical to splicing factor 3b subunit 1 （Sf3bl）, while seven fragments hold the homology of known cDNA clones. In contrast, other two fragments had extremely low identity to any genes registered in GENBANK databases. Conclusion： It is the first time to demonstrate in this study that splicing factor3b, subunitl （Sf3bl） is related to mouse fore stomach carcinoma. Furthermore, ESC-3 and ESC-4 are suggested to contribute to the development of mouse fore stomach carcinoma, thus may be candidates of new targets of oncogenes.
Objective： To screen and identify key genes differentially displayed in mouse fore stomach carcinoma, in order to elucidate the molecular mechanism underlying carcinogenesis. Methods： The animal models complied with each period of NIH mouse fore stomach carcinoma induced by N-Nitrososarcosineethylester （NSEE） were used in this study. The mice were euthanized on days 14, 28, 56, 77 and 84, respectively, after NSEE-piped treatment, and classified according to their pathologies. The differentially expressed genes were isolated from both normal and morbid tissues by mRNA differential display technique and screened by using Reverse Northern blot. Bioinformatics were employed to analyze the results observed. After identification, ten fragments were cloned and matched with GENEBANK database through homologous analysis. Results： One gene was found identical to splicing factor 3b subunit 1 （Sf3bl）, while seven fragments hold the homology of known cDNA clones. In contrast, other two fragments had extremely low identity to any genes registered in GENBANK databases. Conclusion： It is the first time to demonstrate in this study that splicing factor3b, subunitl （Sf3bl） is related to mouse fore stomach carcinoma. Furthermore, ESC-3 and ESC-4 are suggested to contribute to the development of mouse fore stomach carcinoma, thus may be candidates of new targets of oncogenes.
基金
supported by the Zoology Key Subject of Henan Province.
