生脉散组分配伍对热损伤大鼠糖皮质激素受体的影响

Effects of a formula of components from Shengmai Powder on glucocorticoid receptor in rats after thermal injury

目的:研究生脉散组分配伍对热损伤大鼠糖皮质激素受体(glucocorticoid receptor,GR)的影响。方法:雄性SD大鼠32只随机分为正常对照组、模型组、人参总皂苷组和生脉散组分配伍组(简称组分配伍组),每组8只,连续灌胃1周进行预处理。除正常对照组外,其余各组大鼠于末次给药30min后复制大鼠热损伤模型。随后立即断头处死动物,收集血清和肝、肺、肾脏组织。酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测血清皮质酮(corticosterone,CS)的浓度,放射免疫检测法(radioimmu-noassay,RIA)检测血清促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)的浓度;放射性配体受体结合法(radioligand receptor binding assay,RRA)检测肝、肺、肾细胞液GR结合容量。结果:模型组肝、肺、肾胞液GR明显低于正常对照组(P<0.01)。组分配伍组肝和肺胞液GR结合容量明显高于模型组(P<0.01),肾胞液GR结合容量与模型组比较,差异无统计学意义。组分配伍组肝胞液GR明显高于人参总皂苷组(P<0.01),肺和肾胞液GR结合容量与人参总皂苷组比较有上升的趋势,但差异无统计学意义。正常大鼠CS及ACTH水平与模型组、组分配伍组和人参总皂苷组比较,差异有统计学意义(P<0.01),模型组CS及ACTH水平与各给药组比较,差异无统计学意义。结论:生脉散组分配伍可增强人参总皂苷上调热损伤大鼠GR水平的作用。

Objective： To investigate the effects of a formula of components from Shengmai Powder, a compound traditional Chinese herbal medicine, on glucocorticoid receptor （GR） in rats after thermal injury. Methods： A total of 32 male SD rats were randomly assigned into normal control group, untreated group, ginsenosides group and components group, with 8 rats in each group. Rats in the normal control group were intragastrically administered with normal saline （NS） at room temperature once daily. Rats in the untreated group were treated with NS before thermal injury, and rats in the components group and ginsenosides group were once daily treated with a mixture of aqueous extracts of Ophiopogonis Japoni, Fructus schizandrae Chinensis and ginsenosides and ginsenosides respectively. Rats were administered for one week. After the last administration, rats in the untreated group and treated groups underwent thermal injury for one hour, and then were sacrificed immediately by decapitation. Blood serum was collected, and the serum corticosterone （CS） and adrenocorticotropic hormone （ACTH） levels were determined with enzyme-linked immunosorbent assay （ELISA） and radioimmunoassay （RIA） respectively. The liver, lung and kidney homogenates were used to determine the GR binding capacity by radioligand receptor binding assay. The results were analyzed by one point analysis. Results： GR binding capacities in liver, lung and kidney cytosols in the untreated group were obviously lower than those in the normal control group （P〈0. 01）. The GR binding capacities in the liver and lung cytosols in the components group and the ginsenosides group were significantly higher than those in the untreated group （P〈0.01）, but no significant difference was found in kidney cytosol. Compared with the ginsenosides group, GR binding capacity in liver cytosol in the components group was increased （P〈0.01）, but there were no noticeable differences when compared with the GR binding capacities in lung and kidney cytosols. Serum CS and ACTH levels of the normal rats were （66±16） μg/L and （59±18） ng/L respectively. There were significant differences in CS and ACTH levels between the normal control group and the other groups （P〈0.01）, in which the serum CS and ACTH levels were （113±33） μg/L and （125±20） ng/L, （123±26） μg/Land （110±30） ng/L and （118±17） μg/L and （115±35） ng/L respectively. But there was no significant difference between the untreated group and the other treated groups. Conclusion： The formula of components from Shengmai Powder can enhance the effect of ginsenosides in upregulating GR in rats after thermal iniury.