建立更加稳定和有效的兔耳瘢痕模型

Establishment of an animal model for hypertrophic scar

目的:建立更加稳定和有效的兔耳瘢痕模型。方法:选用16只新西兰大耳白兔,分别在兔耳腹侧中段作1cm×1cm的全层皮肤缺损共192个,以形态学、瘢痕增生指数及羟脯氨酸(HPr)的含量变化对创面愈合后形成的增生块,进行动态组织病理学、细胞增殖活性及胶原纤维合成等检测。结果:兔耳腹侧中段创面可产生类似于人类的增生性瘢痕,其发生率为91.5%,增生块最长持续时间可达120多天。结论:采用本实验的模型复制方法,可以得到更加稳定和有效的兔耳增生性瘢痕。

Objective To establish stable and reliable animal model of rabbit ear for hypertrophic scar. Methods Full skin defect （ lcmxlcm in diameter）was created on the ventral side of 32 ears in 16 New Zealand white rabbits.Wound healing under natural condition was observed continuously after the operation. The scar proliferation and index on the rabbit ears were observed by light microscope,the scar HPr content was determined by improved chloraseptine Toxidizing assay. Results The animal model of hypertrophic scar induced by full thickness skin wounds on the ventral side of rabbit ears was similar to that in humans.Its incidence was 91.5 %.The hypertrophic scar could last 120 days. Conclusion Full thickness skin wounds on the ventral side of rabbit ears could produce stable and reliable hypertrophic scars.