ATP敏感性钾通道在乳化异氟醚心肌保护效应中的作用

Contribution of ATP-sensitive K^+ channel to emulsified isoflurane's protective effect on myocardial cells

目的探讨ATP敏感性钾通道(KATP)是否参与乳化异氟醚(EI)对心肌细胞的保护作用。方法原代培养乳鼠离体心肌细胞,建立缺氧/复氧(H/R)损伤模型,随机分为6组,正常组(N组)、缺氧/复氧组(H/R组)、H/R+脂肪乳组(F组)、H/R+10mmol.L-1格列本脲组(G组)、H/R+1.68mmol.L-1EI组(EI组)和H/R+1.68mmol.L-1EI+10mmol.L-1格列本脲组(EIG组),n=6。各组取细胞培养上清液测定LDH活力与cTnI含量,心肌细胞匀浆后测定SOD活力与MDA含量;用倒置显微镜观察心肌细胞生长特性及形态的变化。结果与N组比较,各组的LDH、MDA、cTnI均升高(P<0.05),SOD下降(P<0.05)。与H/R组比较,F组的SOD下降(P<0.05),LDH、MDA、cTnI的差异均无统计学意义(P>0.05),G组的SOD、LDH、MDA、cTnI的差异均无统计学意义;EI组和EIG组的LDH、MDA、cTnI均降低(P<0.05),SOD升高(P<0.05)。与F组比较,EI组和EIG组的LDH、MDA、cTnI均下降(P<0.05),SOD升高(P<0.05)。与EI组比较,EIG组的LDH活力、MDA和cT-nI含量均升高,SOD活力降低(P<0.05)。结论乳化异氟醚对原代培养心肌细胞缺氧/复氧损伤的保护作用可能与其激活ATP敏感性钾通道有关。

Aim To discuss whether the ATP-sensitive K^＋ channel participates in the protective effect of the emulsified isoflurane （EI） on myocardial cells. Method The in-vitro neonate rat cardiac myocytes were primarily cultured, the H/R injury models were established and randomly divided into 6 groups, namely, normal control group （N group）, H/R group （H/R group） , H/R ＋ fat emulsion group （ F group） , H/R ＋ glibenelamide group （ G group） , H/R ＋ 1.68 mmol· L^-1 EI group （EI group）, and H/R ＋ 1.68 mmol· L^-1 EI ＋ 10 mmol · L^-1 ＋ glibenclamide group （EIG group）. Supernatant liquid of cell culture was taken from each group （n = 6） to mensurate LDH and cTnI, and myocardial cell homogenate was used to mensurate SOD and MDA; the changes in the characteristics and forms of myocardial cell growth were observed with inverted microscope. Results Compared to N group, the LDH, MDA and cTnI of other groups all increased （P 〈 0. 05） and SOD declined （ P 〈 0.05）. Compared to H/R group, SOD of F group declined （ P 〈 0.05 ）, and the differences of the LDH, MDA and cTnI had no statistic significance （P 〉 0. 05 ） ; the differences of G group＇s LDH, MDA, SOD and cTnI had no statistic significance （P 〉 0.05） ; LDH, MDA and cTnI of EI and EIG group declined （P 〈0.05） and SOD increased （P 〈 0.05）. Compared to F group, the LDH, MDA and cTnI of EI and EIG group declined （P 〈 0.05） and SOD increased （P 〈 0.05 ）. Compared to EI group, the LDH, MDA and cTnI of EIG group increased （P 〈 0.05 ） and SOD declined （P 〈 0. 05 ）. Conclusion The protective effect of emulsified isoflurane （EI） on the primarily cultured myocardial cells hypoxia/reoxyenation injury may be associated with the ATP-sensitive K^＋ channel activated by it.