摘要
比较了3种从番木瓜组培苗叶片中提取基因组DNA的方法.结果表明:改良CTAB法步骤简单,DNA得率与质量均较高,并能用于转基因植株的PCR检测、限制性内切酶酶切以及后续的反向PCR反应;SDS-KAc法存在RNA残留;试剂盒法的DNA得率与质量均较低.
Three methods for the extraction of genomic DNA from leaves of tissue culture seedlings of Carica papaya L. were com- pared. For the samples ground in the ice bath, genomic DNA with high quality was extracted by the simple processes of modified CTAB method, which could be applied to PCR assay of transgenic papaya, restriction enzyme digestion, and the subsequent inverse PCR. SDS-KAc method was unable to get rid of RNA. DNA extracted by kit showed low production and quality.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2009年第1期34-38,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
福建省自然科学基金资助项目(B0610010)
福建省科技厅资助项目(2008N0008)
关键词
番木瓜
组培苗
基因组DNA
微量提取
Carica papaya L.
tissue culture seedling
genomic DNA
minipreparation
