利尿剂乙酰唑胺在玻碳电极上的伏安行为

Study on the voltammetric behaviour of acetazolamide at glassy carbon electrode

在Britton-Robinson(B-R)缓冲液中,用循环伏安法(CV)、线性扫描伏安法(LSV)和微分脉冲伏安法(DPV)研究了利尿剂乙酰唑胺在玻碳电极(GCE)上的伏安行为.结果表明,在pH6.0的B-R缓冲液中,乙酰唑胺在+1.32 V(vs.SCE)电位处产生一个DPV阳极氧化峰,氧化峰电流与乙酰唑胺浓度在3.00-54.0μmol.L-1范围内呈良好的线性关系,最低检测限为0.450μmol.L-1,从而建立了DPV法定量测定乙酰唑胺的新方法.并初步研究了乙酰唑胺的电化学反应机理.

The electrochemical oxidation of acetazolamide at glassy carbon electrode （GCE） has been studied. The acetazolamide was investigated by cyclic vohammetry （CV）, linear sweep voltammetry （LSV） and differential pulse vohammetry （DPV）, respec- tively. In the Britton-Robinson buffer （ pH 6.0 ）, acetazolamide had a DPV oxidation peak at the potential of ＋ 1.32 V （ vs. SCE） , and the peak current was linearly increased with the concentration of aeetazolamide in the range of 3.00 -54.0 μmol·L-1 , with a detection limit of 0.450μmol·L-1. Thus, DPV method was developed to detect aeetazolamide in synthetic urine and serum samples. The possible mechanism of acetazolamide electrochemical oxidation has been investigated initially.