细胞色素P4503A7＊1c基因多态性与蒙古族妇女骨质疏松的关系

The relationship between CYP3A7 polymorphisms and density in menopausal Mongolian women

目的探讨CYP3A7基因多态性与内蒙古地区蒙古族绝经妇女骨密度的关系。方法应用聚合酶链反应．限制性片段长度多态（PCR-RFLP）测定185名内蒙古地区健康绝经期妇女经SspI酶切后CYP3A7基因多态性，同时用双能X线吸收法测定前臂骨密度。结果基因型频率分布依次为正常组TT型84．4％，TG15．0％，GG0．7％，骨质疏松组TT型71．1％，TG26．3％，GG2．6％，等位基因频率正常组T为91．8％，G为8．2％；骨质疏松组T为84．2％，G为15．8％。通过协方差分析，调整年龄、身高、体重、BMI，结果显示，CYP3A7基因型与左前臂BMD有相关性（P〈0．05）。骨量减少组和骨量正常组间等位基因频率具有显著性差异（P〈0．05），基因型频率无显著性差异（P〉0．05）。结论CYP3A7基因型与蒙古族妇女前臂BMD有关联（P〈0．05），可作为预测骨质疏松发生的危险性因素。

Objective To explore the relationship between CYP3A7 polymorphisms and bone mineral density in menopausal Mongolian women in Inner Mongoha. Methods CYP3A7 polymorphisms defined by the SspI restriction enzyme using polymerase chain reaction-restriction fragment length polymorphism in 185 healthy menopausal Mongolian women in Inner Mongolia, forearm BMD were measured by dual energy X-ray absorptiometry. Results The frequency distribution of CYP3A7 genotype was normal group： TIP 84.4%, TG 15.0%, GG 0.7%; Osteoporosis group： TT 71.1%, TG 26.3 %, GG 2.6 %. CYP3A7 allele frequency were normal group ： T 91.8 %, G 8.2%; Osteoporosis group：T 87%, G 13%, Covarianee analysis showed significant association between CYP3A7 genotype and BMD at left forearm in all groups after adjusting age, body height, body weight and BMI. There was statistical significance between osteopenia group and control group in allele genes, but no statistical significance in genotype frequency. Conclusions Among Mongolian women in Inner Mongolia, CYP3A7 polymorphisms was associated with bone mineral density （P 〈 0.05）. It can be used to predict the risk of osteoporosis.