摘要
目的研究兔前交叉韧带横断术（ACLT）创伤性骨关节炎（OA）动态模型软骨中MMP-1、MMP-9mRNA的表达，探讨MMP01、MMP-9在OA软骨退变中的作用。方法30只新西兰兔随机分为对照组（10只）、ACLT组（20只），ACLT组行右膝ACLT，对照组行关节囊切开缝合术，ACLT组于术后4、8w各处死10只，对照组术后4w处死。解剖显微镜观察股骨内髁软骨形态学变化，逆转录聚合酶链反应（RT-PcR）检测股骨内髁软骨MMP．1、MMP-9mRNA的表达。结果形态学示ACLT组软骨退变重于对照组（P〈0．05），且ACLT8W组重于4W组（P〈0．05）；RT-PCR示ACLT组MMP．1、MMP．9mRNA表达量均高于对照组（P〈0．01），且ACLT8W组均高于4W组（P〈0．01）。结论创伤性OA软骨退变过程中伴随着MMP-1、MMP．9mRNA表达的上调，软骨中MMP-1、MMP．9mRNA的表达一定程度上反映了OA软骨的退变程度。
Objective To observe the mRNA expression of matrix metalloproteinase-1,-9 （MMP-1,MMP-9） in cartilage of traumatic osteoarthritis （OA） in anterior cruciate ligament transaction （ACLT） of rabbit model and to explore the effects of MMP-1 and MMP-9 in Cartilage degeneration of OA. Methods Traumatic OA was induced in 20 rabbits by ACLT of right knee and 10 rabbits killed 4 and 8 weeks respectively after the operation. Other 10 rabbits received right knee arthrotomy as pseudo operative controls and were killed 8 weeks after operation. Cartilagineus changes of the medil femoral condyles were grossly checked and OA severity observed under dissecting microscope. The mRNA expression of MMP-1 and MMP-9 in cartilage of the medil femoral condyles was assessed by Reverse Transcription-Polymerase Chain Reaction （RT-PCR）. Results Cartilage degeneration in ACLT group was significantly severer than that in control group （P 〈 0.05 ）. Cartilage degeneration in 8-week group was also severer than that in 4-week group （ P 〈 0.05）. The expression of MMP-1 and MMP-9 mRNA in ACLT group was significantly higher than those in control group （ P 〈 0.01）. The mRNA expression of MMP-1 and MMP-9 in 8- week group was also higher than those in d-week group （ P 〈 0.01 ）. Conclusions The expression of MMP- 1 and MMP-9 mRNA was up-regulation in cartilage of traumatic OA model. To some extent the mRNA expression of MMP-1 and MMP-9 can be as an evaluation index for the degree of Cartilage degeneration in traumatic OA.
出处
《中国骨质疏松杂志》
CAS
CSCD
2009年第2期96-98,95,共4页
Chinese Journal of Osteoporosis
