蓇密牌钙片阻止OPG基因敲除小鼠的骨量丢失作用

Effects of the GUMIPAI calcium table reverses bone loss in OPG knockout mice

目的研究蓇密牌钙片及鹿角肽对骨保护素（Osteoprotegerin，OPG）基因敲除纯合子小鼠（homozygous OPG knockoutmice，OPG mice）骨质疏松的影响。方法以OPG小鼠为研究对象，钙尔奇D、蓇密牌钙片及鹿角肽分别给各组OPG小鼠连续灌胃30d，酶联免疫法测定血清中抗酒石酸酸性膦酸酶5b（tartrate．resistant acid phosphatase form 5b，TRACPSb）的含量，竞争放射免疫法测定血清中骨钙素（Bone．γcarboxyghtamie acid，BGP）的含量；双能X线骨密度仪（Dual Engergy X—ray Absorbtiometry，DEXA）测定股骨和总体的骨密度（Bone mineral density，BMD）。结果蓇密牌钙片及鹿角肽能增加OPG小鼠的股骨和总体的BMD值，降低TRACPSb的含量，增加BGP的含量。结论蓇密牌钙片对OPG小鼠的骨质疏松症有预防作用，其机制与抑制骨量高转换有关。

Objective To research pharmacodynamic action which GUMIPAI calcium table and the Colla Comus peptide effect on the homozygous OPG knockout mice. Methods The OPG mice is objection, the Caltrate D,the GUMIPAI calcium table,the Colla Comus peptide were used continuously to intragastric administration for 30 days to the OPG mice. The concentration of tartrate-resistant acid phosphatase form 5b （TRACP5b） in serum was measured by enzyme-linked immunosorbent assay; the concentration of Bone-γcarboxyglutamic acid （BGP） in serum was measured by competitive radio immunologic. Bone mineral density （BMD） of the OPG mice was determined by Dual Engergy X-ray Absorbtiometry（DEXA）. Results GUMIPAI calcium table and the Colla Comus peptide can enhance OPG mice thighbone and whole body BMD;the level of TRACP5b was decreased ;the concention of BGP was increased. Conclusions GUMIPAI calium table had antiosteoporotic effects on the OPG mice by inhibiting the bone turnover.