中药注射液血必净对感染性休克犬促凝物质的影响

The therapeutic effects of Chinese injectio xuebijing on coagulant factors in dogs with septic shock

目的研究中药注射液血必净对感染性休克犬促凝物质的影响，探讨血必净治疗感染性休克作用机制。方法在北京朝阳医院基础动物实验室通过给健康杂种犬静脉内注射内毒素制造感染性休克的动物模型，以收缩压下降大于40mmHg开始计时，持续稳定60min，即视为模型成功。按照随机原则将制模的动物分为3组：多巴胺治疗组6只，血必净＋多巴胺治疗组（简称血必净组）6只，另设对照组6只。分别按照实验设计的时间点即注射内毒素前（基础），模型成功后用药前，以及用药后3h检测血管细胞黏附分子（vascular cell adhesion molecule，VCAM）、细胞间黏附分子（inter-cell adhesion molecule，ICAM）、血管性血友病因子（vonwillibrand factor，VWF）和纤溶酶原激活物抑制物-1（plasminogen activator inhibitor-1，PAI-1）血浆含量指标，采用SPSS13.0统计软件进行统计分析。结果犬机体促凝功能监测指标VCAM，ICAM，VWF以及PAI-1：血必净组与对照组、多巴胺组相比，在用药后3h或7h五项监测指标发生了具有统计学意义的变化（P〈0．01或P〈0．05），包括降低同观测点的峰值或者延迟峰值的出现；多巴胺组与对照组相比，ICAM，VWF，PAI-1在3h或7h也出现具有统计学意义的降低（P〈0．01或P〈0．05）。结论在应用多巴胺治疗感染性休克犬的基础上加用血必净可以更加有效地降低感染性休克人体内的促凝物质血浆水平，是中药注射液血必净对感染性休克犬发挥治疗作用的机制之一。

Objective To investigate the therapeutic effects of injectio xuebijing （Chinese herb preparations） on coagulat factors in dogs with septic shock. Method Eighteen dogs infected and shocked by intravenous endotoxin（LPS） were randomly divided into three groups： control group （n = 6） treated with sodium chloride, dopamine group （ n = 6） treated with dopamine, and dopamine plus xuebijing group （ n = 6） treated with dopamine and xuebijing. The therapeutic effects were assessed by comparison of the changes in VCAM （vascular cell adhesion molecule）, ICAM （inter-cell adhesion molecule）, VWF （yon willibrand factor） and PAI-1 （plasminogen activator inhibitor- 1 ） at different indexes including the basic status, the dog being shocked without therapy, 3 and 7 hours after treatment. Analysis was made by SPSS 13.0 software. Results In xuebijing group, VCAM, ICAM, VWF and PAI-1 were significantly changed 3 or 7 hours later after xuebijing administered rather than in control group and dopamine group （ P 〈 0.01 or P 〈 0.05 ）. In dopamine group, ICAM, VWF and PAI-1 were significantly changed 3 or 7 hours after dopamine given rather than in control group （ P 〈 0.01 or P 〈 0.05）. Conclusions Injectio xuebijing obviously decreases the levels of coagulat factors in dogs with septic shock. Therfore, the injectio xuebijing can be used to treat the septic shock.