淫羊藿苷、黄芩苷和大黄素抑制LPS对体外培养人牙龈成纤维细胞的作用

A study of the inhibitory effects of icariin,emodin and baicalin on human gingival fibroblasts(HGF) stimulated by the bacterial endotoxin lipopolysaccharide(LPS)

目的:探讨不同浓度淫羊藿苷、黄芩苷和大黄素对细菌内毒素脂多糖(lipopolysaccharide,LPS)刺激人牙龈成纤维细胞(human gingival fibroblasts,HGF)产生前列腺素E2(prostaglandin E2,PGE2)的影响。方法:以培养的人牙龈成纤维细胞为实验模型,用MTT试验检测淫羊藿苷、黄芩苷和大黄素抑制LPS对牙龈成纤维细胞毒性作用的效果,初步筛选其作用浓度;然后用酶联免疫法检测三种提取物对LPS刺激牙龈成纤维细胞产生重要的骨吸收因子PGE2的影响。结果:LPS对HGF有明显的细胞毒性,三种提取物均可显著提高细胞成活率。LPS作用6h后培养上清中PGE2含量上升,而同时加入淫羊藿苷、黄芩苷和大黄素各浓度组PGE2含量显著低于对照组(p<0.01)。结论:淫羊藿苷、黄芩苷和大黄素均可抑制LPS对HGF的细胞毒性作用,并且可抑制LPS刺激HGF产生PGE2。

Objective： To study the inhibitory effects of icariin, emodin and baicalin on human gingival fibroblasts （HGF） stimulated by the bacterial endotoxin lipopolysaccharide （LPS）. Method： In this protocol, different conditions of emodin, baicalin and icariin were added to the culture of human gingival fibroblasts. MTF assay and enzyme-linked immunosorbent assay were used to examine the effects of these compounds on the inhibitory effects of the proliferation of human gingival fibroblast and the release of PGEz induced by E-LPS. Result： E-LPS show cytotoxity on HGF and, while each of these compounds can increase the cell survival rate significantly （p〈0.01）. The quantity of PGEz of the cell culture media stimulated by E-LPS were significantly higher than added icariin, emodin or baicalin simultaneous （p〈0.01）. Conclusion： The study suggests that the compound of icariin, emodin and baicalin can ameliorate the cytotoxity of E-LPS and inhibit the release of PGE2 by human gingival fibroblasts stimulated with E-LPS.