牙龈卟啉单胞菌菌毛fimA基因在大肠杆菌的融合表达

Fusion expression of the fimA gene of Porphyromonas gingivalis in escherichia coli

目的:克隆牙龈卟啉单胞菌菌毛蛋白fimA基因,并使其在大肠杆菌中融合表达。方法:利用PCR方法克隆fimA,并用基因重组融合表达技术获得在大肠杆菌中的表达。结果:克隆基因测序结果与GenBank数据库中的序列呈现99.9%同源性;经诱导表达后可观察到Mr 38kDa的融合蛋白。结论:成功克隆了fimA基因,并在大肠杆菌中表达了FimA蛋白,为后续研究奠定了基础。

Objective：To clone the fimA gene of Porphyromonas gingivalis （Pg） and detect its fusion expression in Escherichia coll. Method： The desired DNA fragment fimA was obtained by PCR and its expression was used by contruction of fusion protein technique. Result： DNA sequencing showed that the fragment was 99.9% consistent with that of the published. After induction with L-Arabinose, a new 38 kDa protein appeared on SDS-PAGE gel. Conclusion： The fimA gene of Pg was cloned successfully and its protein was expressed correctly in E. coll., and it could be applied for follow-up researches.