TGF-β1,Smad4及Ki-67在胃癌组织中的表达及临床病理意义

Expressions of TGF-β1,Smad4 and Ki-67 in gastric carcinoma and their clinicopathologic significance

目的:探讨转化生长因子β1(TGF-β1)、抑癌基因Smad4和核增殖标志基因Ki-67在胃癌组织中的表达及意义。方法:应用组织芯片、免疫组织化学SP法检测分析60例胃癌、30例非典型增生和20例正常胃黏膜组织中TGF-β1、Smad4和Ki-67的表达。结果:胃癌、非典型增生和正常胃组织中TGF-β1的表达阳性率分别为68.3%、50.0%、45.0%;Smad4的表达阳性率分别为45.0%、66.6%、80.0%;Ki-67的表达阳性率分别为66.6%、53.3%、35.0%。三种标记物的阳性表达在三种组织之间均有显著性差异。结论:胃癌中TGF-β1、Ki-67高表达和Smad4缺失可能共同促进癌细胞的增殖。

Objective： To investigate the expressions and significance of TGF- β1, Smad4 and Ki -67 in gastric carcinoma. Methods： Tissue microarray and immunohistochemical SP method were used to detect the expressions of TGF - β1, Smad4 and Ki - 67 in 60 cases of gastric carcinoma, 30 cases of atypical hyperplasia, and 20 cases of normal gastric mucosa. Results： In gastric mucosa, atypical hyperplasia and normal gastric mueosa, the positive rates of TGF - β1 expression were 68.3% , 50.0% and 45.0% respectively, the positive rates of Smad4 expression were 45.0% , 66.6% and 80.0% respectively, and the positive rates of Ki -67 were 66.6%, 53.3% and 35.0% respectively. The positive expressions of the above three markers among the three groups had significant differences statistically. Conclusion： The overexpression of TGF- [31 and Ki- 67 and the loss of Smad4 in gastric carcinoma may jointly promote the cancer cell proliferation.