穿心莲内酯硫酸酯化反应体系中四个新的衍生物的分离与表征

Isolation and Characterization of Four New Derivatives of Androgra-pholide from Sulfonation System

通过色谱方法,从复杂的穿心莲内酯硫酸酯化反应体系中分离获得4个新的穿心莲内酯衍生物,应用谱学方法进行了表征.主要利用1HNMR,13CNMR,DEPT,1H-1HCOSY,HSQC,HMBC及NOSEY等1D和2DNMR技术,并通过与母体化合物NMR数据的对比分析,鉴定这4个化合物分别为3,19-二羟基-8,11,13-赖百当三烯-15,16-内酯(1),3-羟基-8,11,13-赖百当三烯-15,16-内酯-19-硫酸酯(2),8,11,13-赖百当三烯-15,16-内酯-3,19-二硫酸酯(3),3-羟基-8(R)-12(S)-8(12)-环氧-13-赖百当烯-15,16-内酯-19-硫酸酯(4)。

Four new derivatives of andrographolide were chromatographically isolated from its complicated sulfonation product mixture, and then characterized by spectroscopic methods. Together with 1D and 2D NMR technology, including ^1H NMR,^ 13C NMR, DEPT,^ 1H-^1H COSY, HSQC, HMBC and NOSEY, and comparison of the NMR data with those of the parent compound, the structures of all the four derivatives were elucidated as 3,19-dihydroxy-labdanum-8,11,13-trien-15,16-olide （1）, 3-hydroxy-labdanum-8,11,13- trien-15,16-olide-19-sulfuric ester （2）, labdanum-8,11,13-trien-15,16-olide-3,19-disulfuric ester （3）, and 3-hydroxy-8（R）- 12（S）-8（12）-epoxy-labdanum- 13-en- 15,16-olide- 19-sulfuric ester （4）, respectively.