人参皂甙对手术应激大鼠下丘脑-垂体-肾上腺轴及免疫靶器官中肾上腺皮质激素释放素、ACTH阳性细胞数的影响

The Effect of Ginsenoside on CRF and ACTH Positive Cells in HPAA-spleen of Amputation Stress Rats

目的和方法为探讨手术应激大鼠下丘脑-垂体-肾上腺轴（HPAA）和脾脏中肾上腺皮质激素释放素（CRF）和促肾上腺皮质激素（ACTH）阳性细胞数及人参皂甙的调节作用，采用了免疫酶组化和免疫荧光检测方法。结果应激后15～30min，下丘脑、垂体中CRF和ACTH阳性细胞数较正常对照组减少。CRF阳性细胞在60min后逐渐增多，6h时达正常水平；ACTH阳性细胞于3h开始增多，12h时至正常水平。而肾上腺和脾脏内的CRF和ACTH阳性细胞数在应激后60min开始增多，6h达高峰，24h接近正常水平。人参皂甙（GS）组于应激后15～30min时下丘脑、垂体中CRF和ACTH阳性细胞数多于应激组，但肾上腺和脾脏变化不明显。结论GS可能通过作用于中枢神经内分泌细胞而抑制CAA和ACTH的释放。

Objective To investigate the numbers of corticotropin-releasing factor (CRF) andadrenocorticotropic hormone (ACTH) positive cells in HPAA-spleen of amputation stressrats and the regulation of Ginsenoside (GS). Results The results of immunohistochemicaland immunofluorescent method were shown that the number of the CRF positive cells decreased in hypothalamus and pituitary at 15-30 minutes after amputation, then increased at60 minutes and reached to normal level at 6 hours, while the number of ACTH positive cellsincreased at 3 hours and returned to normal range at 12 hours. In adrenal and spleen, thenumbers of CRF and ACTH positive cells increased at 6 hours and return to normal level at24 hours. In GS experimental group, the numbers of CRF and ACTH positive cells in hypothalamus and pituitary were more than those of in amputation control group. ConclusionThe results suggest that GS may inhibite releasing of CRF and ACTH in hypothalamus andpituitary, and has regulatory effect on activation of HPAA in stress rats.