单核巨噬细胞抗原-1阳性细胞在大鼠肾小球硬化中的表达

Expression of ED1 positive cell in glomerulosclerosis in rats

目的检测单核巨噬细胞抗原（ED）-1在大鼠局灶节段肾小球硬化（FSGS）中的表达，探讨单核巨噬细胞浸润在FsGS发生发展中的作用。方法12只雄性Wistar大鼠，实验组7只，一次性静脉注射氨基核苷嘌呤霉素9mg／100g体重，对照组5只，静脉注射生理盐水一次，动态观察尿蛋白定量变化。第20周末宰杀，检测血浆白蛋白、血脂、肾功能。肾脏制备病理标本。应用免疫组织化学法观察两组大鼠肾组织中纤维连接蛋白（FN）和EDI阳性单核巨噬细胞的表达，计数ED1阳性细胞数，分析其与尿蛋白程度及FN表达的相关性。结果FSGS模型组大鼠用药后尿蛋白定量显著增高，血总胆固醇显著增高。肾脏病理示部分肾小球局灶节段硬化或全球性硬化，细胞外基质节段性增多。在模型组大鼠肾组织中ED1阳性细胞较正常大鼠肾组织显著增多（P〈0．01）。模型组大鼠肾组织中FN的表达较正常大鼠肾组织明显增多（P〈0．05）。ED1阳性细胞数与FN表达呈直线正相关（r=0．876，P〈0．01）。ED1阳性细胞数与尿蛋白程度呈直线正相关（r=0．651，P〈0．01）。结论肾间质单核巨噬细胞浸润可以加重细胞外基质沉积，加重肾小球硬化，提示炎症反应在局灶节段肾小球硬化的发生与发展中起一定作用。

Objective To assay the expression change of ED1 positive cell in focal segmental glomerulosclerosis in rats, we investigated the relationship between the infiltration of mononuclear phagocytes and the progression of glomerular sclerosis. Methods We used 12 Wistar rats which were divided into two groups, test group and control group. The model of focal segmental glomerursclerosis was made by injecting PAN 9 mg/100g body weights. The rats of control group were injected 3ml 0. 9% sodium chloride. The proteinuria, serum creatinine, lipids and protein of the rats were examined. The rats were killed at the 20th week. All the kidneys were kept and made into pathologic sample. Immunohistochemical method was applied to detect the protein expression of FN and the ED1 positive cell in renal tissue of all the rats, and the number of ED1 positive cell was counted. The results were analyzed by SPSS. Results The proteinuria of the rats in FSGS model group was significantly increased, the serum lipid of them was also increased. The pathology changes of the rat renal in model group showed that a part of glomeruli appeared focal segmental sclerosis or all glomerular sclerosis, and the extracellular matrix accumulated. In the renal of model rats, the amount of ED1 positive cells was significantly higher than that in normal rats （ P 〈 0. 05 ）. The expression of FN in the renal of model rats was significantly higher than that in the normal rats （ P 〈0. 05）. The amount of ED1 positive cells was significantly positively correlated with the expression of FN （ P = 0. 002）. The amount of ED1 positive cells was significantly positively correlated with the proteinuria of the rats （ P = 0. 014）. Conclusion The infiltration of ED1 positive cell contributed to the progression of glomerular sclerosis.