甘肃省迭部县莱姆病自然疫源地调查

Investigation on the natural focus of Lyme disease in Diebu county of Gansu province

目的了解实施天然林保护后迭部县莱姆病在人群中的感染现状,自然疫源地的变迁及宿主的莱姆病感染情况。方法人群血清伯氏疏螺旋体抗体的检测采用免疫荧光法,以聚合酶链反应(PCR)检测伯氏疏螺旋体DNA。结果迭部县境内5个林场及7个乡镇采集居民血清522份,莱姆病抗体阳性57份,阳性率10.92%。捕鼠6种69只,平均捕获率15.33%;其中北社鼠25只,占捕获总数的36.23%,褐家鼠18只,占26.09%,大林姬鼠15只,占21.74%,小林姬鼠9只,占13.04%,小家鼠和黑线姬鼠各1只,各占1.45%。对62份鼠脾脏、66份肾脏,用PCR检测伯氏疏螺旋体核酸,结果阳性鼠脾6份,肾5份,鼠携带莱姆病病原阳性率为15.15%(10/66);其中北社鼠6只,褐家鼠2只,大林姬鼠及小林姬鼠各1只。用BSK培养基从采集的鼠肾、膀胱中分离培养出3株伯氏疏螺旋体。结论迭部县广泛存在莱姆病疫源地,人群感染率较高。

Objective To learn the infection status of Lyme disease in Diebu county, the change of natural focus and the situation of the host infected by Lyme disease. Methods The antibody of Borrelia burgdoferi in the crowd serum was detected by immunofluorescence, and the spirochete DNA was tested with PCR. Results A total of 522 serums were sampled from the people in 5 forestry centres and 7 villages of Dieby county, and the antibodies of B.burgdoferi were positive in 57 serums with the positive rate of 10.92%. Sixty nine species of rat-shapes were captured, and the average capture rate was 15.33%. Among them, there were 25 Niniventer confucianus Hodgsons,18 Rattus norvegicus Berkenhouts, 15 Apodemus specious Temminek, 9 Apodemus specious Linnaeus, 1 Mus musculus and 1 Apodemus agrarius Pallas, which accounted for 36.23%, 26.09%, 21.74%, 13.04%, 1.45% and 1.45%, respectively. Six spleens and five kidnies were positive in 62 rats-spleens and 66 rat-kidnies, and the positive rate was 15.15% （10/66）. The positive samples included 6 R.niviventer Hodgsons, 2 R.norvegicus Berkenhouts, 1 A.specious Temminck and 1 A.specious Linnaeus three strains of B.burgdoferi were isolated from rats kidnies and bladders with BSK cuhure medium. Conclusion The natural foci of Lyme disease were widely distributed in Diebu county, and the infection rate in the crowd was higher.