禽胰多肽对肉鸡肝脏和小肠组织中APPR mRNA表达量影响的研究

Effects of Avian Pancreatic Polypeptide on Expression of APPR mRNA in Livers and Small Intestines of Broilers

本试验旨在研究禽胰多肽(APP)对肉鸡肝脏和小肠组织中APPR mRNA表达量的影响。试验以APP粗提液及层析APP制品为材料,选取90只1周龄艾维茵肉鸡随机分为5组,每组3个重复,每个重复6只鸡。Ⅰ设为对照组,Ⅱ、Ⅲ设为饲饮组,Ⅳ、Ⅴ设为注射组。在第7周末,屠宰取其肝脏、小肠组织,运用SYBR Geen Ⅰ实时荧光定量PCR(RT-PCR)法对肝脏和小肠中APPR mRNA的表达进行相对定量分析。结果发现:注射组与饲饮组肝脏及小肠中APPR mRNA的表达水平与对照组相比均呈下降趋势,且注射组降低程度大于饲饮组降低程度;其中,注射组与饲饮组中均是层析APP制品比APP粗提液的下降较明显,提示在外加APP情况下可对APPR mRNA的表达呈现抑制作用。本研究结果为进一步探讨APP与其受体之间的关系,从而为阐明其生理功能和作用机制提供一定的理论依据。

The objective of this study was to investigate the effects of avian pancreatic polypeptide （APP） on expression of avian pancreatic polypeptide receptor （APPR） mRNA in livers and small intestines of broilers. Crude extract liquid of APP and chromatographic APP was used as test material, and ninety 1-week-old broilers were randomly allocated to 5 groups with 3 replicates of 6 broilers. Group Ⅰ was control group; group Ⅱ and group Ⅲ were fed APP; group Ⅳ and group Ⅴ were injected hypodermically APP. After 7 weeks, the broilers were slaughtered randomly in each treatment to determine the relative expression of APPR mRNA in the livers and small intestines of broilers by SYBR Green Ⅰ RT-PCR. The results showed that, compared with control group, the expression of APPR mRNA decreased in the livers and small intestines of the other groups, the decreased degree in injection groups was more significant than that in feed groups; and also the impact of chromatographic APP was more significant than that of crude extract liquid of APP, which suggesting that supplementing APP could inhibitie the expression of APPR mRNA. In conclusion, the above results could provide some theoretical basis for the further research on the relationship between APP and APPR to elucidate physiological function and mechanism.