热-自组装单分子膜表面改性镁的生物相容性

Biocompatibility of surface modified magnesium by heat-self-assembled monolayer

用浸提液法和直接接触发法考察了热-自组装单分子膜表面改性镁及镁钙合金的溶血率、成纤维细胞毒性、成骨细胞毒性。结果表明,pH值<10.61、Mg2+浓度<42mg/L时,纯镁浸提液的溶血率<5%。热-自组装单分子膜改性镁和镁钙合金的耐蚀性提高,其溶血率<5%。成纤维细胞3cm2/ml浸提液稀释到1/3组毒性不符合要求,1/9和1/15组为1级,1/30组的成纤维细胞增殖速度超过阴性对照组,提示低浓度镁浸提液对细胞生长有促进作用。成骨细胞1cm2/10ml浸提液,细胞相对增殖率均在100%以上,细胞毒性为0级;3cm2/ml浸提液中,离子浓度超过382mg/L的1/3稀释组安全性不合格;Mg2+为202mg/L的1/6稀释组满足细胞毒性要求,Mg2+<156mg/L的1/9稀释组对成骨细胞增殖有促进作用。成骨细胞与改性镁和镁钙合金直接接触6d的细胞相对增殖率均在100%以上,未处理镁和镁钙合金增殖率随时间的延长有降低的趋势。

Mg and Mg-Ca alloy were heat treated at 773K for 10h and self-assembled in stearic acid alcohol solution of 0.5mol/L for 1.5h. And then according to ISO 10993.4 standard the hemolysis and cytotoxicity of the Mg and Mg-Ca alloy with and without modification were tested and compared. The results show that the hemolysis rate of the magnesium extract with pH〈10.61 and Mg^2＋〈42mg/L is smaller than 5 %. The hemolysis rate of the modified Mg and Mg-Ca alloy is also smaller than 5% because their resistance corrosion increased. L929 fibroblast and the 1/3 diluted solution of 3cm^2/1ml extract were co-eultured, its cytotoxicity cannot meet the requirement, the cytotoxicity of the 1/9 and 1/15 diluted solution is grade one which meet the requirement, while the fibroblast cell proliferation rate of the 1/30 diluted solution is higher than the negative contrast which hint the extract with low consistency of magnesium ions may promote the fibroblast cell proliferation. Osteoblast eell and the lcm^2/10ml extract were co-cultured, its eytotoxicity is grade zero, relative cell proliferation rate is higher than 100%. The cytotoxieity of the extract with Mg^2＋〉382mg/L cannot meet the requirement, the cytotoxicity of the extract with Mg^2＋ 〈202mg/L can meet the requirement, the extract with Mg^2＋〈 156mg/L may promote the osteoblast cell proliferation After 6 days culture of modified Mg and Mg-Ca alloy direct contact with osteoblast cell the relative cell proliferation rate exceed 100%, while without modified groups have a decrease with time delay.