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猪繁殖与呼吸综合征病毒各结构蛋白的融合表达 被引量：6

Fusion expression of structural proteins of porcine reproductive and respiratory syndrome virus
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摘要 本研究以测序质粒为模板,扩增出结构蛋白基因ORF2a-ORF7,将此基因片段插入真核表达载体pEGFP-N3中构建重组质粒pEGFP-N3-(ORF2a～ORF7),然后将重组表达质粒转染293T细胞。通过荧光显微镜观察显示,重组质粒pEGFP-N3-(ORF2a～ORF7)和载体pEGFP-N3均有绿色荧光出现。SDS-PAGE电泳和western blot分析结果表明重组质粒pEGFP-N3-(ORF2a～ORF7)和载体pEGFP-N3均有EGFP表达;仅ORF7有43ku的融合蛋白EGFP出现,与预期大小相符;其他重组质粒pEGFP-N3-(ORF2a～ORF6)均没有融合蛋白出现。本实验为进一步研究这些蛋白的结构和生物学功能奠定了基础。 In this report, the structural protein genes of porcine reproductive and respiratory syndrome virus （PRRSV） （ORF2a-ORF7） were PCR amplified and cloned into eukaryotic expressing vector pEGFP-N3. The recombinant plasmid pEGFP- N3-（ORF2a-ORF7） was transfected into 293T cell. Fluorescence microscopy showed that EGFP were expressed in all transfected cells. SDS-PAGE and western-blot indicated that only the ORF7 gene was successfully expressed.
作者 曹宗喜 潘全会 张原江 常智杰 罗玉均 何逸民 孔留五 秦宏阳 张桂红
机构地区 华南农业大学兽医学院 清华大学医学院
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2009年第2期85-88,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家高技术研究发展计划(863计划)项目(2006AA10A204) 国家生猪现代农业产业技术体系,广东省农业类计划项目(2007A020300006-4)
关键词 猪繁殖与呼吸综合征病毒 结构蛋白 真核表达 PRRSV structural protein eukaryotic expression
分类号 S852.659.6 [农业科学—基础兽医学]
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参考文献12

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二级参考文献2

共引文献7

同被引文献54

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引证文献6

二级引证文献10

中国预防兽医学报
2009年 第2期

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