大孔树脂对射干萃取液中鸢尾苷物质的提取

Study on the Separation and Purification of Tectoridin from the Extract of Rhizoma belamcandae with Macroporous Resin

研究了几种大孔吸附树脂的静态吸附特性,筛选出一种吸附性能较好的XDA-1大孔吸附树脂,并应用于柱层析进行射干正丁醇萃取液的分离纯化。研究了萃取液中鸢尾苷物质浓度、流量及温度等参数对大孔吸附树脂吸附性能的影响。对操作条件的优化得到了满意的实验结果:XDA-1大孔吸附树脂对鸢尾苷物质的静态吸附率达到83.48%,柱层析用体积分数70%乙醇洗脱,洗脱率达到93%。在一般室温下,操作温度对大孔树脂的吸附效果影响较小。

By compare the absorption static characteristic of several macroporous resin, the XDA-1 macroporous resin was selected and applied to separated and purify Tectoridin from the n-butanol residual extract of Rhizorna belamcandae. The effect of absorption parameters, such as concentration of tectoridin, flow rate of solvent and temperature, was investigated in this study. Through optimizing the operation conditions, a series of excellent results was achieved： the absorption rate of the resin was 83. 48%, 70% ethanol was used as washout solvent, the washout rate was 93% . Furthermore, the normal room temperature has no effect on the absorption. There is no much effect on absorption at normal room temperature.