深黄被孢霉高产花生四烯酸菌株的微波诱变育种

Breeding of Arachidonic acid Producting Strain with Mortierella sabellina by Microwave Mutation

为获得生长活力较强、产ARA能力强的菌株,以干菌重、微生物油脂产量和花生四烯酸(ARA)产量为评价指标,采用两轮微波诱变的方法,利用单因素试验确定微波累计加热时间,并采用气相色谱分析ARA含量。试验结果表明:微波频率为高档,累计加热时间为35 s,其致死率为78.3%。经过两轮微波诱变及菌种筛选,获得一株高产菌株W35s2-153,其生物量为30.85 g/L,总油脂含量为15.5 g/L,ARA质量浓度为2.61 g/L,ARA产量比原始对照菌株提高3.18倍,并且遗传性能稳定。

The aim of this study was to screen a high growth vigor, high biomass concentration, high ARA production strain. For this, two twice microwave mutation （hypso-step and 35 s heating time） was adopted, the dried mycilia, microbes olein yield, and RAR yield of the mutants were determined to select the aim mutant. Strain W35s2-153, which arachidonic acid content was higher 3. 18 times than that of the parent, was screened. The mutants exhibited high genetic stability.