珍珠草乙肝舒康胶囊药效学研究

Pharmacodynamic Research on HBV Recovery Capsule Prepared with Common Leafflower

目的:探讨珍珠草乙肝舒康胶囊抗HBV及保护肝细胞的药效学作用及其特点。方法:通过体外2.2.15细胞株实验观察珍珠草乙肝舒康胶囊抑制HBV的效果,建立大鼠CCl4慢性肝损伤模型,分为空白对照组,CCl4组,乙肝宁组,珍珠草乙肝舒康大、中小剂量组,检测各组生化及肝左叶病理学组织差异;参照文献方法,测定小鼠腹腔巨噬细胞吞噬功能及小鼠血清溶血素含量,观察珍珠草乙肝舒康对小鼠免疫功能的影响。结果:HBsAg,HBeAg的最大抑制率为76.47%(d7),71.43%(d5),且呈现HBV分泌、复制越是旺盛,抑制作用越强的特点;珍珠草乙肝舒康组与模型组相比,肝功能明显改善,肝组织结构破坏轻,纤维组织增生程度轻,与乙肝宁组相比,各项指标变化略占优势;小鼠腹腔巨噬细胞吞噬功能增强,羊红细胞致敏的小鼠血清溶血素含量提高,显著优于空白对照组,与阳性对照组盐酸左旋咪唑片相似。结论:珍珠草乙肝舒康胶囊具有抗HBV、保护肝细胞作用。

Objective：to conduct a pharmacodynamic research into HBV recovery capsule prepared with Common Leafflower about its anti-HBV and protective effect of liver cells. Methods： anti- HBV effect was observed through in vitro experiment in 2.2.15 cell line. Rat model of chronic liver injury was established using CC14, and was divided to blank control group, CC14 group, Yigan Ning Group, and Leafflower high-dose group, middle-dose group and low-dose group. Each group was subject to biochemical examination and histo- logical examination to pathological changes in left hepatic lobe; peritoneal macrophage phagocytosis and content of haemolysin were determined in mice to observe the capsule＇ s effect on immune function. Results ： maximum inhibition ratio of HBsAg and HBeAg were re- spectively 76.47% （d7） and71.43% （d5）, moreover, HBV secretion and replication correlated positively with inhibitive action. When compared with CC14 group, the leafllower group improved significantly in liver function, with less severe liver disorganization and fibro- plasias; and was superior over various parameter value to Yigan Ning group. The leafflower group had similar effect as Levamisole, which was better than the blank control group： phagocytosis of peritoneal macrophage was reinforced, and content of haemolysin increased following sensitization induced by sheep red blood cell. Conchtsion：HBV recovery capsule prepared with Common Leafflower had anti-HBV effect and protection of liver cells.