补充CO2对光生物反应器培养新月菱形藻的影响

Effects of CO_2 Enrichment on Growth and Photosynthesis of Nitzschiaceae closterium

为了优化光生物反应器培养微藻的条件,研究了在充空气的基础补充CO2对光生物反应器培养新月菱形藻(Nitzschiaceae closterium)生长和光合作用的影响。实验表明,补充CO2(含1000μL/L CO2的空气)促进新月菱形藻的生长,藻细胞密度和生物量显著高于对照组(CO2含量350μL/L)(P<0.05)。补充CO2也能够提高藻细胞叶绿素a和类胡萝卜素的含量(P<0.05),但是对叶绿素b没有显著影响(P>0.05)。补充CO2能够显著提高指数生长期的最大光合速率(Pm)、光合作用效率(α)和光合作用饱和光强(Ik)(P<0.05)。结果表明,CO2是光生物反应器培养微藻的限制因子之一,补充CO2能够提高微藻的生物量。

In order to optimize the parameter, the effects of CO2 enrichment on the growth and photosynthesis of Nitzschiaceae closterium in air lift photobioreactors were investigated. The results showed that cell density and biomass of N. closterium significantly increased when culture mediun was bubbled with enriched CO2 （1 000 μL/L） compared to low CO2 concentration （350 μL/L）. Moreover, CO2 enrichment increased the Chla and Car content of algae cell, and had no significantly effect on the Chlb content of algae cell. The maximum photosynthesis rate （Pm）, the initial slope （a） and saturating irradiance level （Ik） in exponential growth phase rose with CO2 enrichment. The results show that CO2 is one of limiting factors for algae growth. The biomass of Nitzschiaceae closterium is improved by enriched CO2.