3种市售养殖对虾白斑综合征病毒(WSSV)的核酸探针检测

White Spot Syndrome Virus (WSSV) Detection with Nucleic Probe in Three Cultured Shrimps

对虾白斑综合征病毒是对虾养殖业危害最为严重的病毒,每年给对虾养殖造成很大经济损失,已成为对虾养殖业可持续发展的严重障碍。在过去的十几年中,人们采取各种方法防止白斑综合征病毒的传播。中国明对虾(Fenneropenaeus chinensis)、凡纳滨对虾(Litopenaeus vannamei)、日本囊对虾(Marsupenaeus japonicus)是我国主要的对虾养殖品种,也是白斑综合征病毒的敏感宿主。按照煮沸-乙醇沉淀法快速、简便提取市售30只中国明对虾、28只凡纳滨对虾、29只日本囊对虾DNA,然后用地高辛标记的核酸探针进行斑点杂交检测白斑综合征病毒,从而了解市场中养殖对虾携带白斑综合征病毒的情况。检测结果显示,所有样品均未感染白斑综合征病毒,说明目前白斑综合征病毒在一定程度上得到了有效控制。

White spot syndrome virus （WSSV） was considered the most serious virus for shrimp aquaculture. This virus has brought great yearly economic losses in world shrimp farming and has become a major deterrent in the growth and sustainability of shrimp aquaculture. In the past more than 10 years, many techniques have been used to prevent this virus＇ transmission. Fenneropenaeus chinensis, Litopenaeus vannamei, Marsupenaeus japonicus are three kinds of popular cultured shrimp in China. They are also WSSV acute hosts. In this paper, DNA of 30 Fenneropenaeus chinensis, 28 Litopenaeus vannamei, 29 Marsupenaeus japonicus bought from seafood market were extracted rapidly by applying the method of boiling - ethanol precipitation, and then detected by digoxigenin - labeled nucleic probe dot blot hybridization for white spot syndrome virus carrying status. The results showed that all of the samples were WSSV negative, which indicated that WSSV was controlled effectively to some degree in China.