青海省几个主要春性甘蓝型油菜杂交种的SSR分析

Analysis of spring rapeseed hybrids and their parental lines in Qinghai Province by SSR markers

为了探明青海省主要甘蓝型油菜品种的遗传变异,建立准确高效的种子纯度鉴定方法,本研究选取5个青海省甘蓝型油菜主栽品种,利用140对简单重复序列(Simple sequence repeat,简称SSR)引物对其进行分子标记分析,从中筛选出26对能清晰扩增出多态性主带的引物,进一步对包括这5个品种及其中4个杂交品种的7个亲本系进行扩增后,经6%聚丙烯酰胺凝胶电泳分离和带型分析,成功构建出5个青海省甘蓝型油菜主栽品种及其中4个杂交种亲本系的指纹图谱,并鉴定出了这4个杂交种的共显性标记。这些标记不仅可用于亲本材料和杂交品种真伪的鉴别,还可为杂交种纯度的鉴定提供可靠方法,对亲本知识产权的保护及杂交种推广具有重要的意义。

In order to identify the genetic diversity and establish the technology for seed purity inspection, five spring rapeseed varieties （Brassiea napus L. ） mainly planted in Qinghai province and seven parental lines of hybrid varieties,were selected for simple sequence repeat （SSR） analysis. After screening 140 pairs of SSR primers,26 pairs were selected for their polymorphism and further utilized in this study. Gel electrophoresis of the amplified product was carried out with 6% polyacrylamide, and DNA finger printing of the materials was successfully constructed. In addition, co - dominant markers of the four hybrids were identified. These markers can be used not only to distinguish false seeds and identify the factuality of their parent lines, but also to supply reliable methods for hybrid seed purity inspection, which is of great importance for the property protection of the parental lines and the safe popularization of hybrid seeds.