SDS—聚丙烯酰胺凝胶电泳分析壳寡糖

Electrophoretic Analysis of Chitooligosaccharide by SDS-PAGE

目的研究一种快速准确的分析壳寡糖组成的定性测定方法。方法通过连续电泳和不连续电泳对比实验确定壳寡糖的最佳分析条件。结果浓缩胶浓度为3%,分离胶浓度为17.5%,上样量为10μl,恒定电流为40mA,电泳时间50min条件下,获得最佳壳寡糖分析图谱。结论SDS-聚丙烯酰胺凝胶电泳分析壳寡糖组成的方法的确是一种简单快速有效地定性检测方法。

Objective To explore a qualitative, fast and accurate way for analyzing composition of chitooligosaccharide. Methods By consecutive electrophoresis and non-consecutive electrophoresis experiments, the best analysis conditions of chitooligosaccharide were determined. Results The concentration of plastic was 3 percent, the concentration of separation of plastic was 17.5 percent, the volume of samples was 10ul, constant current was 40 mA , electrophoresis time was 50 min. Best analysis photos of chitooligosaccharide Can be obtained under those conditions. Conclusion The method of analyzing composition of chitooligosaccharide is truly a simple, effective, accurate and qualitative way.