猪囊尾蚴病特异诊断方法研究 Ⅱ.ABC-ELISA法

STUDIES OF DETECTING THE ANTIBODY TO CYSTICERCUS CELLULOSAE WITH ABC-ELISA METHODS

对囊尾蚴寄生较少、体液抗体量甚微的病猪,其它方法不容易检测出来,应用ABC-ELISA法,即亲和素-生物素复合物(Aridin Biotin Compiex)酶标法,借助其放大作用,大大提高了灵敏度。经试验测定,其灵敏度高于ELISA法的9～14倍,并且可将EL-ISA法判定的疑似猪得到分化,从而提高了符合率。

By using Aridin-Biodin Complex (ABC) test, we detected the anti-body to cysticercus cellulosae which iffeced the disease. artificially and naturally, and the ABC test was compared with the indirect ELISA test. The results show that the AEC test has magnifying function in addition to all the advantages that the ELISA test pessesses. Yet ihe AEC test can determine positive or negtive sera that gave uncertain results in the ELISA test.Therefore,the AEC test is considered to be a very specific and highly sensitive method in detecting the trace antibody to cysticercus cellulosae in pigs.