高效液相色谱法测定食品中合成色素金黄粉

Determination of Acid Orange Ⅱ in Foods by HPLC

采用VP-ODS-C18(250L×4.6)色谱柱,以甲醇-醋酸铵水溶液为(0.02mol/L)流动相,流速1.0mL/min,梯度洗脱,柱温30℃,在221nm波长处用紫外检测器检测食品中合成色素金黄粉含量。金黄粉在3.0—100.0mg/L浓度范围内与峰信号呈良好的线性关系,A=5.607C-1.322(r=0.9998),检出限为1.11mg/L。该方法灵敏、简便,其重复测定结果的精密度和准确度较好,可用于食品中合成色素金黄粉的测定。

Acid orange Ⅱ in foods was determined by HPLC with the stationary phase of VP-ODS-C18（250L×4.6）,gradient elution＇methyl alcohol and 0.02mol/L ammonium acetate as the mobile phase,and UV detector at 221nm at flow rate of 1.0mL/min and 30℃.The calibration curve is linear within 3.0—100.0mg/L with the equation of A=5.607C-1.322（r=0.9998）,the minimum detection limit was 1.11mg/L.This method is rapid,accurate,reliable and simple,and can be used to determine the acid orangeⅡ in foods.