峨嵋岩白菜叶和根状茎总黄酮提取工艺研究

Study on Extraction Technology of Total Flavonoids in Bergenia Emeiensis C.Y.Wu Leaves and Rhizomes

采用微波辅助提取法,以总黄酮含量为考察指标,通过单因素实验和正交试验优选出峨嵋岩白菜叶和根状茎总黄酮微波最佳提取工艺。实验结果表明,峨嵋岩白菜叶中总黄酮提取的最佳工艺条件为:乙醇浓度60%,料液比1∶30,微波时间60s/次,微波功率480W,提取次数为3次,黄酮含量为15.181mg/g;根状茎中总黄酮最佳工艺条件为:乙醇浓度60%,料液比1:20,微波时间75s/次,微波功率480W,提取次数为2次,黄酮含量为2.066mg/g。峨嵋岩白菜叶中总黄酮含量较根状茎中丰富,微波辅助提取法适合峨嵋岩白菜有效成分的提取。

The total flavonoids in Bergenia emeiensis C.Y.Wu leaves and rhizomes was extracted with microwave-assisted extraction（MAE）.By using single factor test and orthogonal experiment,the optimization technology of microwave-assisted extraction of flavonoids in Bergenia emeiensis C.Y.Wu leaves and rhizomes were obtained.The optimization conditions were：60% ethanol,solid-liquid ration of 1∶30 for 3 times,60s each time,and 480W as the microwave power,the contents of flavonoids were 15.181mg/g,the optimization technology for the flavonoids in B.emeiensis rhizomes were：60% ethanol,solid-liquid ration of 1∶20 for 2 times,75s each time,and 480W as the microwave power,the contents of flavonoids were 2.066mg/g.The contents of flavonoids in B.emeiensis leaves are more than those in B.emeiensis rhizomes,and the microwave-assisted extraction method is reasonable to extract chemical constituents in B.emeiensis.