大肠埃希菌老年患者分离株β-内酰胺酶基因与毒力基因研究

β-Lactamase Genes and Virulence Genes in Escherichia coli Isolated from Old Patients

目的了解大肠埃希菌(ECO)老年患者分离株β-内酰胺酶基因与毒力基因产生状况。方法对20株ECO进行15种β-内酰胺酶基因(TEM、SHV、CTX-M-1群、CTX-M-2群、CTX-M-9群、OXA-1群、OXA-2群、OXA-10群、PER、GES、VEB/CEF、CARB、DHA、ACT/MIR、LAT/CMY)及毒力基因(CNF-1、CNF-2)检验。结果20株ECO检出TEM基因14株、SHV基因3株、CTX-M-1群基因1株,20株ECO中至少检出1种β-内酰胺酶基因,7株同时检出>2种β-内酰胺酶基因。结论该20株ECOβ-内酰胺类抗菌药物耐药与产β-内酰胺酶密切相关。

OBJECTIVE To investigate the β-laetamase genes and virulence gene in Escherichia coli isolated from the old patients. METHODS Twenty strains of E. coli were selected to test 15 kinds of β-lactamase genes （TEM, SHV, CTX-M-1 group, CTX-M-2 group, CTX-M-9 group, OXA-1 group, OXA-2 group, OXA-10 group, PER, GES, VEB/CEF, CARB, DHA, ACT/MIR and LAT/CMY） and two virulence genes （CNF-1 and CNF-2）. RESULTS Six β-lactamase genes and one virulence gene have been found out from these 20 strains of E. coll. The results showed that there were 14, 3, 1, 5, 2, 2 and 1 strain which carried TEM, SHV, CTX-M-1 group, OXA-1 group, DHA, ACT/MIR and CNF-1, respectively. These 20 E. coli strains contained at least 1 β-lactamase gene and 7 E. coli strains were found containing more than 2 kinds of β-lactamase at the same time. CONCLUSIONS The resistance of these 20 E. coli strains to β-lactam antibiotics is closely relative to β-lactamase.