生长激素和胰岛素对猪卵母细胞体外成熟及孤雌胚卵裂的影响

Effects of STH and Insulin on Porcine Oocytes IVM and Oocytes Cleavage

本研究探讨了生长激素(STH)和胰岛素对猪卵母细胞体外成熟及孤雌激活后卵裂的影响。结果表明:在mNCSU-23液中,添加0.15μg/mLSTH组的成熟率(73.83%)和卵裂率(64.76%)显著高于对照组和添加0.01、0.05、0.1、0.2μg/mLSTH组(P<0.05);添加5μg/mL和8μg/mL胰岛素组的成熟率(66.25%、60.64%)显著高于添加0、0.5、2μg/mL及10μg/mL组(P<0.05);添加5μg/mL胰岛素组的卵裂率(61.63%)显著高于添加0、0.5、2、8、10μg/mL组(P<0.05);最佳的联合添加组为0.15μg/mLSTH+2μg/mL胰岛素,其体外成熟率为79.37%、卵裂率为72.31%。

This experiment investigated the effects of STH and insulin on porcine oocytes maturation in vitro and cleavage after parthenogenetic activation. The results showed that the maturation rate （73.83%） and the cleavage rate （64.76%） derived from oocytes matured in mNCSU-23 supplemented with 0.15 ug/mL STH were significantly higher than those supplemented with 0.01, 0.05, 0.1, 0.2 ug/mL and control group （P 〈 0.05）. When 5 ug/mL or 8 ug/mL insulin was added to mNCSU-23, respectively, the maturation rate （66.25%, 60.64%） were significantly higher than that of other groups（P 〈 0.05）, and the cleavage rate（61.63%） matured in mNCSU-23 supplemented with 5 ug/mL insulin was significantly higher than that of other groups （P 〈 0.05）. The optimum concentrations for STH and insulin to use together were 0.15 ug/mL and 2 ug/mL, the rate of maturation and cleavage were 79.37%, 72.31%, respectively.