运动训练对亚硝酸钠致记忆障碍模型小鼠学习记忆能力的影响

Effects of exercises on learning and memory deffict induced by sodium nitrite in mice

目的:考察运动训练对亚硝酸钠致记忆障碍模型小鼠学习记忆能力的影响,并探讨其作用机制。方法:昆明种小鼠采用被动跑轮法进行运动训练。训练6周后,采用小鼠Morris水迷宫法测试小鼠学习记忆能力;比色法检测大脑组织中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,HE染色观察海马组织形态学改变。结果:与空白对照组比较,模型对照组小鼠定向游泳实验潜伏期显著延长(P<0.05),SOD活力显著降低(P<0.05),MDA含量显著升高(P<0.05),海马神经元变性、脱落;与模型对照组比较,运动组小鼠游泳潜伏期显著缩短(P<0.05),SOD活力显著增强(P<0.05),MDA含量显著降低(P<0.05),海马神经元的变性及脱落显著改善。结论:运动训练能显著改善亚硝酸钠所致小鼠学习记忆障碍,其作用机制可能与提高小鼠自由基清除能力有关。

Objective： To investigate the effects of exercises on learning and memory deffiet induced by sodium nitrite in mice and determine the mechanism preliminarily. Method： After 6 weeks of exercise with the running wheels water maze task was used to evaluate learning and memory function of mice. The activity of SOD and content of MDA were measured with colorimetry. The morphology alterations in hippoeampus slices were assessed under microscope. Result：As compared with control group, in model group the escape latency in directional swimming prolonged and SOD activity decreased and content of MDA increased. Compared with model group, in exercise group, the escape latency shortened, SOD activity increased, the content of MDA decreased,neuro degenerative changes improved and the death of neuron in hippocampus slices reduced. Conclusion： These results suggest that exercises has improving effects on learning and memory deffict induced by sodium nitrite in mice. The mechanism may be related to decreasing of free radical.