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非洲猪瘟病毒环介导等温扩增快速检测方法的建立 被引量：34

Establishment of a Loop-mediated Isothermal Amplification Assay for the Detection of African Swine Fever Virus
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摘要 本试验利用环介导等温扩增技术(LAMP)建立了一种快速高效检测非洲猪瘟病毒的方法。整个反应在水浴锅中恒温进行,不需要其他昂贵仪器,30 min即可得到阳性结果,灵敏度是OIE标准PCR方法的100倍,并且与其他常见猪DNA病毒无交叉反应。该方法非常适合在野外现场或缺乏试验条件的边远地区检测非洲猪瘟病毒。 A rapid assay for the African swine fever virus （ASFV） by loop mediated isothermal amplification （LAMP） was established. This method was performed in a water bath at 63 ℃ without any other expansive equipment. Positive results were got in 30 min with a sensitivity 100 fold of PCR. And no cross-reaction with other usual DNA virus of swine was observed. This method is used suitablely to detect ASFV in field or any other place lack of a laboratory environment.
作者 江彦增 朱鸿飞
机构地区 中国农业科学院北京畜牧兽医研究所
出处 《中国畜牧兽医》 CAS 北大核心 2009年第2期72-74,共3页 China Animal Husbandry & Veterinary Medicine
关键词 非洲猪瘟病毒 环介导等温扩增 African swine fever virus LAMP
分类号 S852.651 [农业科学—基础兽医学]
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参考文献8

  • 1孙怀昌.非洲猪瘟病毒研究进展[J].中国预防兽医学报,2006,28(1):117-120. 被引量：31
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二级参考文献22

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共引文献55

同被引文献351

引证文献34

二级引证文献273

中国畜牧兽医
2009年 第2期

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