实验性大鼠乳腺增生的细胞核形态计量学研究

Nuclear Morphometric Studies of Experimental Mammary Gland Hyperplasia in Rats

应用图像分析技术对大鼠乳腺轻度增生、增生活跃、不典型增生及正常乳腺组织进行细肥核形态学计量研究，检测4项参数，即细胞核面积、周长、最长径和形态因子。结果显示，轻度增生与正常乳腺间无显著性差异（P＞0．05）；增生活跃组与正常组间、不典型增生组与增生活跃组间均有显著或非常显著的差异（P＜O．05，P＜O．01）。表明随着乳腺增生程度的加重，细胞核增大，核形状变异，将增加乳腺癌的危险性。

Nuclear morphometry of the slight hyperplastic epithelim,the active hyperplastic epithelium,the atypical hyperplastic epithelium and the normal epithelium of the mammary gland in rats were observed by image analysis system. 4 morphometric parameters(area, perimeter,longest diameter and shape factor)of the cell nuclei were determined.The results showed that there are significant or great significant difference among the active hyperplasia,the atypical hyperplasia and the normal mammary epithelium (P＜0.05, P＜0.01), and no significant difference between the slight hyperplasia and the normal mammary gland (P＞0.05).Thus,we suggest that with increasing of the hyperplastic degree of mammary epithelium,the nuclei volume will enlarge,the nuclei shape will variate,and the risk of breast cancer will increase.