干扰素和卡介苗对肝癌大鼠单核巨噬细胞释放IL—1的影响

Effect of IFNα-2b and BCG on the Release of IL-1 by Monocytes and Macrophages in RAT with Hepatoma

以二乙基亚硝胺（DEN）诱发大鼠肝癌，正常大鼠和肝癌大鼠分别腹腔注射干扰素（IFNα—2b）、卡介苗（BCG）或二者联用。从正常大鼠和肝癌大鼠体内分离出高纯度的血单核细胞、肺、脾和腹腔内的巨噬细胞，体外分别与人肝癌细胞联合培养，测定培养液中的IL—1的活性。此外，尚在肝癌大鼠体内分离出高纯度的血单核细胞、肺、脾和腹腔内的巨噬细胞，体外受IFNα—2b、BCG或二者联合作用后，同上测定培养液中的IL—1的活性。结果显示：IFN0α—2b或BCG均可促进上述单核巨噬细胞释放IL—1，并均以两者联用的效果最佳，四种单核巨噬细胞释放IL—1为对照组的1．09～2．04倍不等。本结果提示肝癌病人进行免疫治疗时，应考虑两种免疫制剂联合应用。

Hepatoma was induced in rats by administration of diethylnitrosamine(DEN).The normal rats and hepatoma rats were treated controls were not treated w.ith any immune stimulants(IS). The peritoneal macrophages, lung and spleen macrophages, monocytes were isolated from normal and hepatoma rats (at 8th, 12th and 16th week) and cocultured with human SMMC-7721 hepatoma cell,the activity of IL-1 in media were measured by 3TdR incorporation of, thymocytes isolated from mice; the peritoneal macrophages, lung and spleen macrophages, monocytes were isolated from control hepatoma rats(at 18th week)and treated with IS in vitro, and were then cocultured with human SMMC-7721 hepatoma cell,the activity of IL-1 in media were assayed. The results showed that both in normal and hepatoma rats, either in vivo or in vitro, IFNα-2b and BCG enhanced monocytes and macrophages to release IL-1, particully,in combination with IFNα-2b and BCG groups which increased the production of IL—1 by 0.09~1.04 times.The results suggested that the combined application of IS to patients suffering from hepatoma may be much better than the iS used alone.