原发性肝癌Plk1基因表达及其与预后的相关性

Expression of gene Plk1 and its relationship with prognosis of hepatocellular carcinoma

目的:分析Plk1基因在肝细胞癌(hepatocellular carcinoma,HCC)中的表达,及其与预后的关系.方法:用半定量RT-PCR及Western blot方法检测我院2003-01/2008-05原发性肝癌患者213例的Plk1基因蛋白表达,同时应用Kaplan-Meier法及多变量Cox比例险模型,分析Plk1基因的表达及临床病理因素与预后的关系.结果:Plk1基因在肝癌组织中阳性表达率为83.6%.Plk1阳性组的1、3、5年的生存率明显低于阴性组(P=0.004).原发性肝癌的预后与Plk1阳性表达,Edmondson分级,肉眼癌栓,显微癌栓及肿瘤数目相关;与HBsAg、肝硬化、AFP、假包膜、肿瘤大小无关;多因素Cox回归分析显示Edmondson分级、Plk1基因蛋白表达、肉眼癌栓及肿瘤数目4项指标反映肝癌预后情况,危险度分别为1.717、1.938、1.537及2.355.结论:Plk1基因的检测有助于有高危肝癌患者的选择,为Plk1基因靶向治疗肝癌提供了有力依据.

AIM： To investigate expression of polo-like kinasel （Plkl） in hepatocellular carcinoma （HCC） tissue and to explore relationship between Plkl expression and prognosis of HCC. METHODS： Protein expressions of Plkl were detected using semi-quantitative RT-PCR and Western blot in 213 HCC cases at our hospital from Jan 2003 to May 2008. And clinicopathologic factors and prognosis were analyzed by the application of Kaplan-Meier method and multivariate Cox proportional hazards model. RESULTS： The positive expression rate of Plkl in HCC was 83.6%. 1, 3, 5-year survival rates were significantly lower in positive expression group than in negative group （P = 0.004）. The prognosis of HCC was correlated with the positive expression of Plkl, Edmondson grade, macroscopic venous invasion, microscopic venous invasion and tumor number in HCC, but not with HbsAg, cirrhosis, AFP, tumor encapsulation or tumor size. In multivariate Cox regression analysis, Edmondson grade, the expression of Plkl, macroscopic venous invasion and tumor number were four important prognostic factors for HCC. The risk ratio of Edmondson grade, positive expression of Plkl, macroscopic tumor thrombosis and tumor number were 1.717, 1.938, 1.537, 2.355, respectively. CONCLUSION： The expression of Plkl would be helpful in selecting the high risk cases and providing reliable evidence for Plk1 gene targeted therapy.