植物药洪天肝康防治肝硬化的作用机制

Prevention and treatment of hepatic cirrhosis with herbal medicine Hongtian Gankang

目的:探讨植物药洪天肝康防治肝硬化的作用机制.方法:采用200mL/LCCl4腹腔注射制备小鼠肝硬化模型(模型组),于模型制备的同时,给予洪天肝康灌服(预防组).造模150d后添加洪天肝康灌服(治疗组).取各组的肝、脾组织,采用HE染色观察肝硬化程度及肝、脾组织的形态学变化;免疫组织化学方法标记TGF-β1、IFN-γ、IgG和CD3,并用图像分析仪进行定量分析.结果:模型组小鼠的肝脏及脾脏TGF-β1的表达较对照组明显增高,预防组及治疗组肝脏与脾脏中TGF-β1的表达均较模型组减少(F=80.155,120.850;均P<0.05);IFN-γ和IgG在肝脏及脾脏的表达以治疗组最为明显,预防组次之,模型组极少出现(F=53.584,118.24085.075,105.566,均P<0.05);预防组及治疗组CD3在肝脏及脾脏的表达明显增高,与模型组相比有统计学意义(F=42.969,69.775,均P<0.05).结论:植物药洪天肝康对肝硬化模型小鼠机体进行动态平衡的整体调节后,可增强机体的免疫防御功能,从有效控制肝纤维化产生的角度,有效地防治肝硬化.

AIM： To investigate the mechanism underlying preventing and treating hepatic cirrhosis with Hong Tian Gan Kang （HTGK） in mice. METHODS： Sixty mice were divided randomly into 4 groups： normal control group, model control group, prevention group and treatment group. Except the mice in normal control group, all mice were given injection of CCl4 into abdominal cavity that had been proved to induce experimental hepatic cirrhosis. In prevention group, mice were also given HTGK simultaneously by intragastric administration for 180 days, whereas mice in treatment group were given HTGK 150 days after modeling. Liver tissue and spleen tissue were made into sections stained with HE to evaluate the extent of liver cirrhosis and morphologic changes and to detect the expression of TGF-β1, IFN-γ, IgG, CD3 using immunohistochemistry. RESULTS： Expression level of TGF-β1 in liver and spleen was markedly higher in model group than in control, prevention and treatment groups （F = 80.155, 120.850, both P 〈 0.05）. The expression of IFN-γ, and IgG was most obvious in treatment group, second in prevention group and least in model group （F = 53.584, 118.240, 85.075, 105.566, all P 〈 0.05）. Compared with model group, expression levels of CD3 in prevention and treatment groups were significantly increased （F = 42.969, 69.775, both P 〈 0.05）. CONCLUSION： HTGK can improve the function of immune system so that it can prevent and cure hepatic cirrhosis effectively.