同源异形盒基因CDX2的表达与胃黏膜肠上皮化生类型的关系

Relationship between the expression of CDX2 and different types of gastric intestinal metaplasia

目的:探讨CDX2表达与胃黏膜肠化生类型的关系.方法:利用免疫组化技术检测58例正常胃黏膜、184例肠化生和36例胃癌组织CDX2表达;利用HID-ABpH2.5-PAS将184例肠化生分为Ⅰ型81例,Ⅱ型62例和Ⅲ型41例.结果:正常胃黏膜CDX2阴性表达,胃癌组织CDX2表达高于正常胃黏膜(P<0.01),低于肠化生黏膜(59.8%vs27.8%,P<0.01);Ⅰ型、Ⅱ型肠化生CDX2表达高于胃癌和Ⅲ型肠化生,差异均有统计学意义.唾液酸黏蛋白着色的Ⅰ型肠化生CDX2呈清晰着色,硫酸黏蛋白着色的Ⅲ型肠化生CDX2未见着色.结论:CDX2可作为判断肠化生分化程度的指标.

AIM： To inquire the relationship between the expression of CDX2 and different types of gastric intestinal metaplasia. METHODS： Expression of CDX2 in 58 normal gastric mucosa, 184 IM and 36 gastric cancer were detected using immunohistochemical method. One hundred and eighty four IM cases were classified into three subtypes, including 81 cases of IM Ⅰ, 62 cases of IM Ⅱ and 41 cases of IM Ⅲ using HID-ABpH2.5-PAS methods. RESULTS： The positive rates of CDX2 expression in IM and gastric cancer were significantly higher than normal gastric mucosa in which negative expression was detected （P 〈 0.01）, but lower than IM （27.8% vs 59.8%, P 〈 0.01）. The positive rates of CDX2 expression in IM Ⅰ and IM Ⅱ were significantly higher than either gastric cancer group or IM Ⅲ However, there was no significant difference between IM Ⅲ and gastric cancer group. CDX2 was located in IM cellular nucleus. CONCLUSION： The IM without expression of CDX2 has close relationship with gastric cancer, and CDX2 is a valuable biomarker to judge the differentiation of IM.