应用RT-PCR法检测肝细胞癌的HCV表达

Expression of HCV in Hepatocellular Carcinoma Detected by PCR Technique

目的研究肝细胞癌的发生与丙肝病毒感染的关系。方法应用聚合酶链式反应对肝细胞癌(55例)和肝内胆管结石的肝组织(21例)的石蜡包埋标本中的HCV-RNA和HBV-DNA进行检测。结果肝癌组和结石组的HCV-RNA阳性例数分别为18例(32.7%)和1例(4.8%),OR=9(P<0.05)。结论HCV感染是HCC的重要致病因素,用PCR技术检测石蜡包埋肝组织中的HCV-RNA是一种简便、特异、灵敏的方法。

Objective To investigate the relationship of HCV infection to the onset of hepatocellular carcinoma. Methods HCV-RNA and HBV-DNA were detected by polymerase chain reaction （PCR） technique in formalin-fixed paraffin-embedded （FFPE） liver tissue specimens from 55 Chinese patients with HCC and 21 with hepatolithiasis serving as controls. Meanwhile,we reviewd the ELISA results testing immuneological markers of HBV and HCV in the patients and controls. Results HCV-RNA was present in 10（32.7%） samples in HCC group and 1 sample（4.8%） in controls.The OR（odd ratio） value for HCC associated with positive HCV-RNA was 9（P〈 0.05）. Furthermore,in liver tissues of HCC the positive incidence of HBV-DNA was 56.7% and there was no significant relationship between HCV-RNA positive and HBV-DNA positive in HCC patients （P 〉 0.05 ）. Conclusion HCV infection is an important pathogenic factor of HCC. Using RT-PCR technique in the detection of HCV-RNA in FFPE liver tissue is a simple, specific and sensitive method.