福辛普利、氯沙坦对自发性高血压大鼠脂肪组织脂联素的影响

Effects of fosinopril and losartan on adiponectin expression in adipose tissue of spontaneously hypertensive rats

目的:探讨福辛普利和氯沙坦对自发性高血压(SHR)大鼠脂肪组织脂联素的影响。方法:22周龄SHR大鼠随机被分成四组,分别予福辛普利、氯沙坦、福辛普利加氯沙坦、安慰剂喂养。同系魏凯氏(WKY)大鼠作对照组。8周后取尿检测大鼠尿微量蛋白(Upro)和n-乙酰β-d-氨基葡萄糖苷酶(NAG)含量,取脂肪组织用免疫组化法及逆转录聚合酶链式反应(RT-PCR)检测脂联素的mRNA的表达。结果:SHR大鼠脂肪组织脂联素表达较WKY大鼠降低;Upro和NAG酶水平升高(P均<0.01);福辛普利组和氯沙坦组与SHR组比较血压降低(P<0.01);Upro和NAG酶水平降低(P<0.01),脂联素表达上调(P<0.01)。结论:(1)SHR大鼠Upro和NAG酶水平升高,脂肪组织脂联素表达下调;(2)福辛普利和氯沙坦可以提高SHR大鼠脂肪组织脂联素表达,降低Upro和NAG酶水平。

Objective： To observe the impacts of fosinopril and losartan on adiponectin expression in adipose tissue of vascular walls of spontaneously hypertensive rats （SHR）. Methods： Twenty two weeks old male SHR were randomly divided into four groups： Model （SHR） group, fosinopril （Fos） group, losartan （Los） group and fosinopril＋losar-tan （Fos＋Los） group; Five 22-week old male Wistar-Kyoto rats （WKY） were regard as the control group. After 8 weeks, 24 h urinary protein （Upro）, urinary N-acetyl-β-d-glucosaminidase （NAGase） were detected; The mRNA ex- pression of adiponectin was detected by RT--PCR; the protein expression of adiponectin was tested by immunohisto chemistry. Results： As compared to WKY group, 24h urinary protein content and urinary NAGase were increased, adiponectin expression in adipose tissue of vascular walls was down-regulated in SHR groups （P〈0. 01 all） ; as com- pared to SHR group, blood pressure was lowered, 24h urinary protein content and urinary NAGase decreased, adi- ponectin expression was up-regulated in Fos, Los and Fos＋ Los group （P〈0. 01 all）. Conclusion： Urinary protein content and urinary NAGase increased, adiponectin expression in adipose tissue of vascular wails is down-regulated in SHR group; Fosinopril and/or losartan can decrease urinary protein content and urinary NAGase, up--regulate adi-ponectin expression in adipose tissue of vascular walls of SHR.