红外光谱法对肉苁蓉径向不同部位的分析与评价

Analysis and Evaluation of Different Radial Part of Cistanche Deserticola by Fourier Transform Infrared Spectroscopy and Two-dimensional Infrared Correlation Spectroscopy

采用傅里叶变换红外光谱、二阶导数谱和二维相关红外光谱技术对肉苁蓉由表及里3个部位的药材粉末及其水提物和醇提物进行了分析与评价研究。结果表明,肉苁蓉不同部位的一维光谱非常相似,三者相似系数分别为0.9605,0.944和0.976;二阶导数谱中峰位和峰强的差异明显。1430～1700cm-1范围内的二维相关谱中皮部自动峰有3个,而中部及髓部均为4个,更直观的反映出三者的差异。不同部位水提物和醇提物的分析结果进一步明确了肉苁蓉皮部芳香类、环烯醚萜类及糖苷类物质与中部和髓部存在明显不同,而髓部的水溶性多糖、半乳糖醇和苯乙醇苷类物质均高于其它部位。可见红外光谱法结合二维相关红外光谱技术为同种药材不同部位的细微差异分析和评价提供了一种快速、全面和客观的方法和手段。

Fourier transform infrared spectroscopy （FTIR） combined with two-dimensional correlation spectroscopy （2D-IR） was used to analyze and evaluate the different parts of Cistanche deserticola. The crude drugs of Cistanche deserticola and their different extracts were both referred. The results of the analysis showed that single-dimensional FTIR of the different parts from Cistanche deserticola were very similar, where the similarity coefficients are 0. 9605, 0. 944 and 0.976, respectively. Moreover, the shape and intensity of the peaks were more distinct in the secondary derivative IR spectra of the different parts. The fingerprint characters of the different parts in 2D-IR spectra were more visualized. In the range of 1430 - 1700 cm^-1 , 3 strong autopeaks were aroused in the cortex, but 4 autopeak in the middle and central parts. The spectra of aqueous and alcoholic extracts from different parts of Cistanche deserticola showed that aromatic compounds, iridoids and indieans in the cortex were different from that of the middle and central parts. As well, the contents of water-soluble polysaccharide, galactitol and phenylethanoid glycosides in the central part were higher than the other parts. Therefore, FTIR combined with 2D-IR was an express and comprehensive approach to analyze and evaluate the imperceptible differences among different parts of the medicinal herbs impersonally.