郏县红牛细胞色素B序列测定及多态性分析

Sequence Analysis and Polymorphism Analysis of Cytochrome B in ‘JiaXian Red Cattle’

以郏县红牛基因组DNA为模板,对mtDNA Cytb全序列进行扩增、测序、序列核苷酸组成分析、序列的变异和多态性分析,得出2点结论:①mtDNA基因全序列长度均为1 140 bp,不存在序列长度差异,富含A和T。②全序列比对与普通牛仅有1个突变位点,与瘤牛有8个突变位点。

Using genome DNA of ＇Jiaxian Red Cattle＇ as template, the mt DNA Cyt b was amplified, sequenced, and analyzed to nucleotide composition. Two conclusions have been drawn： the first one is that mt DNA has the same length of 1140 base pairs; and the second one is that there is one mutation compared with bos. Taurus and there are 8 mutations compared with zebu cattle.