肌腱细胞类胰岛素生长因子-1受体密度分析

ANALYSIS OF THE DENSITY OF INSULINLIKE GROWTH FACTOR1 RECEPTOR ON TENDON CELL

为了了解类胰岛素生长因子１（ＩＧＦ１）受体在不同代次体外培养的肌腱细胞的分布，同时观察同一肌腱细胞周期中不同亚时相的ＩＧＦ１受体数目的差别，采用免疫组化和流式细胞仪分析法，对体外培养的原代、第６代和第１３代肌腱细胞的ＩＧＦ１受体密度进行了分析，并以第６代肌腱细胞为对象，进行了同一细胞周期不同亚时相的ＩＧＦ１受体数目差别分析。结果表明，ＩＧＦ１受体在体外培养的原代、第６代和第１３代肌腱细胞的密度大体相同，而在同一细胞周期中分裂前期及分裂期（Ｇ２Ｍ期）的受体数目比ＤＮＡ合成前期（Ｇ１期）的受体数目多（Ｐ＜０．０１）。提示，在肌腱细胞培养的传代过程中和在同一细胞周期的不同亚时相，肌腱细胞均维持一个相对稳定的ＩＧＦ１受体密度。

For the purpose of understanding the distribution of insulinlike growth factor1(IGF1) receptor on the tendon cell, the continuous cultured tendon cell line was studied by following experiments.With the methods of immunohistochemical study and flow cytometric study, the density of IGF1 receptor of the primary, 6th and 13th generation of tendon cell was analyzed. The results showed that there was no difference of the receptor density among those generations. However, in the cell cycle, the numbers of IGF1 receptor in G2M phase tendon cells were more than that in G1 phase cells(P<0.01). These works provided sufficient evident which suggested there were stable density of IGF1 receptor on the tendon cell though out the life span of tendon cell. This may build some foundation in growth control of tendon cell by growth factor in the research of tendon tissue engineering.