商陆皂甙甲对阿霉素致肾小球硬化大鼠TGF-β_1表达的影响

Effect of Esculentoside A on the expression of TGF-β_1 in rats with glomerulosclerosis induced by adriamycin

目的观察商陆皂甙甲对阿霉素致肾小球硬化大鼠TGF-β1表达的影响及其可能机制。方法采用单侧肾切除并阿霉素尾静脉注射的方法建立大鼠肾小球硬化模型。将48只SD大鼠随机分为假手术组(N组)、模型组(M组)、商陆皂甙甲组(S组)、依那普利组(Y组),分别给予生理盐水、商陆皂甙甲、依那普利治疗,共100 d。观察商陆皂甙甲对肾小球硬化大鼠肾小球病理改变及24 h尿蛋白、肾功能的影响。用免疫组化方法测定肾小球TGF-β1的表达。结果术后第7,13周S组大鼠24 h尿蛋白排泄量、处死前血肌酐(SCr)、尿素氮(BUN)水平、肾小球硬化指数显著低于M组(P均<0.01),肾小球病理损害轻于M组,血清白蛋白较M组显著增高(P<0.01)。结论商陆皂甙甲可下调肾小球硬化大鼠肾小球TGF-β1的表达,减少细胞外基质的沉积,进而保护肾功能,延缓肾小球硬化的发生、发展。

Objective It is to observe the effects of Eseulentoside A （EsA） on the expression of TGF- β1 in adriamycininduced Glomerulosclerosis rats and to explore its possible mechanisms. Methods The glomerulosclerosis rat models were established by adriamycin intravenous injection from caudal vein combined with one kidney cutting off. Forty-eight male SD rats were randomly divided into four groups： sham-operated group （group N）, model group （group M）, EsA group （group S）, and Enalapri[ group（group Y）, and given respective drugs for 100 d. The expression of TGF - β1 in glomeruli was detected by immunohistochemistry method. The levels of 24 h urinary protein excretion and SCr, BUN and pathological changes in renal tissue were observed at the same time. Results At the end of 7th, 13th week after operation, the levels of 24 h urinary protein excretion and SCr, BUN before death in group S were significantly lower than those in group M（ P 〈 0. 01 ）, furthermore, the pathological damage was lighter and level of serum albumin was higher（ P 〈 0.01 ）. Conclusion Esculentoside A can inhibit over-expression of TGF-β1 in glomeruli decrease ground substance ecto-deposition, so it can protect renal function and inhibit the development of glomerulosclerosis in rats with glomerulosclerosis.