反向高效毛细管电泳法测定乙酰水杨酸制剂含量

Determination of Aspirin in Preparation Form by Capillary Electrophoresis

以十六烷基三甲基溴化铵为电渗流改向剂，建立了适合乙酰水杨酸制剂优化分离与定量分析的反向高效毛细管电泳法。电泳分离条件：熔融石英毛细管４０ｃｍ×５０μｍ，ｕｎｃｏａｔｅｄ（河北永年），缓冲液组成为０．０５ｍｏｌ／Ｌ磷酸盐溶液（ｐＨ５．０），含０．５ｍｍｏｌ／Ｌ电渗流改向剂和５．０ｍｍｏｌ／Ｌβ－环糊精，运行电压１１ｋＶ（－）→（＋），柱上检测ＵＶ２１０ｎｍ，０．０２ａｕｆｓ。以磺基水杨酸为内标，测得乙酰水杨酸浓度在０．０５～０．２５ｍｇ／ｍｌ范围内与样品峰面积比的线性关系良好，相关系数ｒ＝０．９９９４，平均回收率为９７．２２％，日内ＲＳＤ低于０．９６％，日间ＲＳＤ低于２．６％。

A high performance capillary electrophoresis method with reversed direction of electroosmotic flow induced by cationic surfactant was established for the separation and determination of aspirin in preparation form A buffer solution composed of 005 mol/L sodium dihydrogenphosphate at pH 50 containing 05 mmol/L cetyltrimethylammonium bromide and 50 mmol/L βcyclodextrin was found to be the suitable electrolyte analysis The electromigration was performed on the 40 cm×50 μm uncoated capillary with a 11 kV runining voltage The analytes were monitored by on line ultraviolet (UV) detection at 210 nm and sulfosalicylic acid was selected as internal standard The method was simple, rapid and well reproducible The line regression range was between 005￣025 mg/ml, recovery was 9722% and the intraday precision was 096%, interday precision was 26%