雌二醇诱致大鼠垂体催乳素瘤形成过程催乳素基因DNase　Ⅰ敏感位点增加

The PRL Gene is Highly Sensitive to Digestion by DNase I in theChromation of Anterior Pituitary Tumors Induced by Estradiol

ＤＨａｓｅⅠ敏感位点的存在，是染色质水平上基因活化调节的重要基础之一。为探讨Ｅ２诱发垂体ＰＲＬ瘤形成的表基因机制，本工作观察了Ｅ２诱致的垂体催乳素瘤催乳素基因ＤＮａｓｅⅠ敏感位点的改变。结果表明：Ｅ２诱致大鼠垂体前叶ＰＲＬ瘤细胞ＰＲＬ基因的ＤＮａｓｅⅠ敏感位点较正常大鼠增多，提示在Ｅ２诱发ＰＲＬ瘤形成过程中，ＰＲＬ基因处于活化状态。

The present work determined whether there was. a change in the state of DNase I sites in the chromatin of anterior pituitary tumors induced -by estradiol in SD rat. by Southern blot hybridization. The results indicated that the PRL gene in the chromatin of anterior pituitary tumors induced by estradiol was more sensitive to digestion by DNase I than the control group. This suggested that PRL gene in the chromatin of anterior pituitary tumors induced by estradiol was in a more active state than the normal anterior pituitary.